Embryo rescue in wide crosses in Arachis. 3. In vitro culture of peg tips of A. hypogaea selfs and interspecific hybrids

Moss, J.P. and Stalker, H.T. (1987) Embryo rescue in wide crosses in Arachis. 3. In vitro culture of peg tips of A. hypogaea selfs and interspecific hybrids. Peanut Science, 14 (2). pp. 70-74.

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Abstract

A. hypogaea cv. NC4 was either self-pollinated or pollinated by the tetraploid amphidiploid A. duranensis X A. chacoense. The resulting pegs were cultured 4, 10 or 20 days after pollination with and without meristems, and in the presence or absence of gibberellic acid (GA). Pegs elongated in culture only when the peg meristem was not removed. Ovules enlarged and grew out of the surrounding peg tissue in 3.8 to 32.8% of the cultures. Significantly more ovules grew when the peg meristem was removed and when 10 and 20- day-old pegs were cultured. Overall, the most successful treatment for growth of ovules was treating flowers with GA at pollination and culturing without the peg meristem 10 days after pollination when 25.0 and 32.8% of all hybrid and selfed ovules, respectively, grew. Embryo growth was observed in an average of 8.4 and 17.6% of embryo sacs in hybrid and self peg tips, respectively, with several embryos reaching the globular stage after 21 days in vitro

Item Type: Article
Author Affiliation: Department of Crop Science, North Carolina State University, Raleigh, NC 27695, USA
Subjects: Plant Production
Crop Improvement > Plant Breeding
Divisions: Groundnut
Depositing User: Mr B Krishnamurthy
Date Deposited: 27 Apr 2011 04:49
Last Modified: 27 Apr 2011 04:49
URI: http://eprints.icrisat.ac.in/id/eprint/1895

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