A method for isolation and purification of peanut genomic DNA suitable for analytical applications

Sharma, K.K. and Lavanya, M. and Anjaiah, V. (2000) A method for isolation and purification of peanut genomic DNA suitable for analytical applications. Plant Molecular Biology Reporter, 18. 393a-393h.

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Numerous methods are available for isolation of plant genomic DNA, but in practice these procedures are empirical due to variability in plant tissue composition. Consistent isolation of quality DNA from peanut (Arachis hypogaea L.) is particularly problematic due to the presence of phenolic compounds and polysaccharides. Inconsistencies in extraction results can be attributed to the age and growth stage of the plant material analyzed. Mature leaves have higher quantities of polyphenols, tannins, and polysaccharides that can contaminate DNA during isolation. We show that four published protocols could not be used to isolate peanut DNA of sufficient quality for PCR amplification or Southern hybridization. We have devised a new protocol that uses DEAE-cellulose purification to isolate peanut DNA useful for downstream applications

Item Type: Article
Author Affiliation: Genetic Transformation Laboratory, Genetic Resources and Enhancement Program, International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Patancheru, Andhra Pradesh 502 324, India
Subjects: Crop Improvement
Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Crop Improvement > Biotechnology
Divisions: Groundnut
Depositing User: Mr T L Gautham
Date Deposited: 05 May 2011 10:24
Last Modified: 05 May 2011 10:25
URI: http://eprints.icrisat.ac.in/id/eprint/1975

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