RNA sequencing: advances, challenges and opportunities

Ozsolak, F. and Milos, P.M. (2011) RNA sequencing: advances, challenges and opportunities. Nature Reviews Genetics, 12 (2). pp. 87-98.

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In the few years since its initial application, massively parallel cDNA sequencing, or RNA-seq, has allowed many advances in the characterization and quantification of transcriptomes. Recently, several developments in RNA-seq methods have provided an even more complete characterization of RNA transcripts. These developments include improvements in transcription start site mapping, strand-specific measurements, gene fusion detection, small RNA characterization and detection of alternative splicing events. Ongoing developments promise further advances in the application of RNA-seq, particularly direct RNA sequencing and approaches that allow RNA quantification from very small amounts of cellular materials

Item Type: Article
Author Affiliation: Helicos BioSciences Corporation, One Kendall Square, Cambridge, Massachusetts 02139, USA
Subjects: Crop Improvement > Genetics/Genomics
Divisions: General
Depositing User: Syamala
Date Deposited: 11 May 2011 04:59
Last Modified: 11 May 2011 04:59
Official URL: http://dx.doi.org/10.1038/nrg2934
URI: http://eprints.icrisat.ac.in/id/eprint/2014

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