New Saccharum hybrids in S. spontaneum cytoplasm developed through a combination of conventional and molecular breeding approaches

Pan, Y.B. and Burner, D.M. and Wei, Q. and Cordeiro, G.M. and Legendre, B.L. and Henry, R.J. (2004) New Saccharum hybrids in S. spontaneum cytoplasm developed through a combination of conventional and molecular breeding approaches. Plant Genetic Resources: Characterization and Utilization, 2 (2). pp. 131-139.

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Abstract

Identification of sugarcane F1 hybrids is difficult when selections are based solely on morphological traits. Our objective was to combine morphological traits and molecular marker analysis to select F1 hybrids from 2 separate crosses between Djatiroto, a clone of S. spontaneum, and elite sugarcane clones, LCP 85-384 (Cross 97-3144) and CP 62-258 (Cross 97-3146). The maternal inflorescences of Djatiroto were emasculated by submersion in a circulating 45°C hot-water tank for 10 minutes to minimize self-fertilization. Cross 97-3144 produced 4.7 g of seeds with 338 viable seeds/gram and Cross 97-3146 produced 2.4 g of seeds with 166 viable seeds/gram. After greenhouse germination, 96 progeny from each cross were evaluated in a field plot. Evaluations were conducted on the ratoon crops for stalk diameter (mm), juice Brix (percentage soluble solids), and a randomly amplified polymorphic DNA (RAPD) marker OPA-11-366 that was reproducibly amplified through PCR from the elite clones, but not the maternal S. spontaneum clone. Fifty progeny (52.1%) from Cross 97-3144 and 36 progeny (37.5%) from Cross 97-3146 inherited the RAPD marker. Five putative F1 progeny were selected from each cross, namely US 99-43, US 99-44, US 99-45, US 99-46 and US 99-47 from Cross 97-3144, and US 99-48, US 99-49, US 99-50, US 99-51 and US 99-52 from Cross 97-3146, based on their relatively larger stalk diameter, higher Brix and inheritance of the RAPD marker. The hybrid nature of these selected progeny was verified with sugarcane microsatellite markers. This is thought to be the first report of the development of Saccharum hybrids with the cytoplasm of S. spontaneum for breeding purpose through a combination of conventional and molecular breeding approaches

Item Type: Article
Author Affiliation: Sugarcane Research Unit, Southern Regional Research Center, USDA-ARS, 5883 USDA Road, Houma, LA 70360, USA
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: Other Crops
Depositing User: Syamala
Date Deposited: 19 May 2011 08:06
Last Modified: 19 May 2011 08:06
URI: http://eprints.icrisat.ac.in/id/eprint/2044

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