Improved screening techniques for in-vitro seed colonization and pre-harvest seed infection by Aspergillus flavus in groundnut

Thakur, R.P. and Rao, V.P. and Upadhyaya, H.D. and Nigam, S.N. and Talwar, H.S. (2003) Improved screening techniques for in-vitro seed colonization and pre-harvest seed infection by Aspergillus flavus in groundnut. Indian Journal of Plant Protection, 31 (2). pp. 54-60.

[img] PDF
Restricted to ICRISAT researchers only

Abstract

Pre-harvest infection of groundnut seeds by toxigenic strains of Aspergillus flavus Link ex Fries and A. parasiticus Speare is a major cause for aflatoxin contamination.The objective of this study was to refine screening techniques fo in-vitro seed cilonization and pre-harvest seed infection that could be effectively used in resistance breeding programs.A highly aggressive and toxigenic strain of A flavus,Af11-4 was used to improve the screening techniques for in-vitro seed colonization and pre-harvest seed infection. The in-vitro seed colonization technique invilved thr use of sodium hypochlorite (NaOCl-clorax 20%)instead of mercuric chloride (HgCl2 O.1%)or ethanol(ethyl alcohol 70%)as seed surface disinfectant and multiwell dishes to incubate single inoculated seed that facilitated selection of seeds resistant to A.flavus colonization. The pre-harvest seed infection screening technique involved development of A.flavus infested beds and imposing soil moisture strees (54-63% waterdeficit)during the last 48 days of the crop growth.Both in-vitro seed colonization and field screening methods were successfully used to screen a number of groundnut genotypes with consistent results.

Item Type: Article
Author Affiliation: International Crops Research Institute for Sem-Arid Tropics,Patancheru 502 324,Andhra Pradesh,India
Subjects: Plant Protection
Divisions: Groundnut
Depositing User: David T
Date Deposited: 18 Jul 2011 10:32
Last Modified: 18 Jul 2011 10:32
URI: http://eprints.icrisat.ac.in/id/eprint/2249

Actions (login required)

View Item View Item