Transformation of microbes, plants and animals by particle bombardment

Klein, T.M. and Arentzen, R. and Lewis, P.A. and Fitzpatrick-McElligot, S. (1992) Transformation of microbes, plants and animals by particle bombardment. Bio/Technology, 10 (3). pp. 286-291.

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Recent developments with particle bombardment systems are described. The Biolistic Particle Delivery System has been refined to use a shock wave generated by the sudden release of compressed helium PDS-1000/He to accelerate a thin plastic sheet carrying microprojectiles into a metal screen, with a lowering of tissue damage. A hand-held version of the PDS-1000/He has been used to deliver genes to tissues of living animals. Slight changes in bombardment parameters can influence greatly the level of gene transfer. The use of the technique is considered for the following: (1) stable transformation of plants (including 'recalcitrant' species); (2) transient assays of gene expression; (3) gene transfer to organelles (including yeast mitochondria, Chlamydomonas chloroplasts and tobacco plastids); (4) gene transfer to microbes (including Bacillus megabacterium and Magnoportha grisea); and (5) gene transfer to animal cells and tissues (animal cells in vitro, egg/embryo bombardment, gene expression in organ explants, gene transfer to live animals)

Item Type: Article
Author Affiliation: DuPont Agricultural Products, Experimental Station, 402/4250, Wilmington, DE 19880, USA
Subjects: Plant Protection > Pesticides
Soil Science and Microbiology
Soil Science and Microbiology > Microbiology
Divisions: General
Depositing User: Mr B Krishnamurthy
Date Deposited: 25 Jul 2011 04:45
Last Modified: 25 Jul 2011 04:45
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