High-velocity microprojectiles for delivering nucleic acids into living cells

Klein, T.M. and Wolf, E.D. and Wu, R. and Sanford, J.C. (1987) High-velocity microprojectiles for delivering nucleic acids into living cells. Nature, 327 (6117). pp. 70-73.

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A method is described in which DNA or RNA is delivered into plant cells using small tungsten particles (microprojectiles; 4 µm diameter) at high velocity. After acceleration, the particles pierce the cell walls and membranes and enter the intact cells without killing them. Using this method, 1 cm² sections of Allium cepa epidermis were bombarded with tungsten particles coated with plasmid p35S-CAT, containing a gene that encodes chloramphenicol acetyltransferase (CAT). Extracts subsequently obtained from the tissue revealed expression of the gene, in the form of very high levels of CAT activity; controls showed negligible activity. It is suggested that the method can be used to study the transient expression of foreign genes in intact tissue and can possibly provide a broadly applicable means of transformation capable of circumventing the host range restrictions of Agrobacterium tumefaciens and the regeneration problems associated with protoplasts

Item Type: Article
Author Affiliation: Department of Horticultural Science, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, USA
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: General
Depositing User: Mr B Krishnamurthy
Date Deposited: 25 Jul 2011 04:35
Last Modified: 25 Jul 2011 04:35
URI: http://eprints.icrisat.ac.in/id/eprint/2446

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