A simple and general method for transferring genes into plants

Horsch, R.B. and Fry, J.E. and Hoffmann, N.L. and Eichholtz, D. and Rogers, S.G. and Fraley, R.T. (1985) A simple and general method for transferring genes into plants. Science, 227 (4691). pp. 1229-1231.

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Surface-sterilized leaf discs from petunia, tobacco and tomato were inoculated with an Agrobacterium tumefaciens strain containing a modified Ti plasmid with a chimaeric gene for kanamycin resistance. The discs were cultured for 2 days and then transferred to a selection medium containing kanamycin. Shoot regeneration occurred within 2 to 4 weeks, and transformants were confirmed by their ability to form roots in medium containing kanamycin. This method thus combines gene transfer, plant regeneration and selection for transformants in a single step

Item Type: Article
Author Affiliation: Biological Sciences, Monsanto Company, St. Louis, Missouri 63167, USA.
Subjects: Crop Improvement > Genetics/Genomics
Divisions: General
Depositing User: Mr B Krishnamurthy
Date Deposited: 25 Jul 2011 04:34
Last Modified: 25 Jul 2011 04:34
URI: http://eprints.icrisat.ac.in/id/eprint/2448

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