Quantitative determination of phytate and inorganic phosphorus for maize breeding

Lorenz, A.J. and Scottb, M.P. and Lamkey, K.R. (2007) Quantitative determination of phytate and inorganic phosphorus for maize breeding. Crop Science, 47 (2). pp. 600-6004.

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Abstract

Phytate is the dominant storage form of phosphorus (P) in mature cereal and oil grains. Phosphorus bound in phytate is nutritionally unavailable to monogastric animals and thus contributes to water pollution because it is excreted in the waste. Also, phytate can chelate certain minerals and exacerbate human mineral deficiencies. Our primary objective was to develop a rapid and inexpensive method of measuring phytate and inorganic P (Pi) concentrations in maize (Zea mays L.). The procedure reported herein was derived from previously published assays and used to screen 50 inbred lines to determine its potential in a selection program. Grain yield, protein, oil, methionine, lysine, tryptophan, and kernel weight were also measured. Field repeatability values for phytate and Pi (0.78 and 0.91, respectively) suggest that our protocol can be used to make heritable measurements on both traits. Phytate measurements taken with the procedure reported herein matched closely those obtained through ion exchange. The combination of adequate precision and simplicity make this method ideal for breeders interested in improving Pi and phytate levels simultaneously. The positive phytate:protein correlation reported commonly was also detected in this study. A relationship between phytate and kernel weight indicates that selection for low phytate may result in larger kernels

Item Type: Article
Author Affiliation: Department of Agronomy, University of Wisconsin–Madison, Madison, WI 53706
Subjects: Crop Improvement
Divisions: Maize
Depositing User: Ms K Syamalamba
Date Deposited: 17 Aug 2011 08:20
Last Modified: 17 Aug 2011 08:20
Official URL: http://dx.doi.org/10.2135/cropsci2006.03.0177
URI: http://eprints.icrisat.ac.in/id/eprint/2576

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