An in vitro method for identifying postattachment Striga resistance in maize and sorghum

Amusan, I.O. and Rich, P.J. and Housley, T. and Ejeta, G. (2011) An in vitro method for identifying postattachment Striga resistance in maize and sorghum. Agronomy Journal, 103 (5). pp. 1472-1478.

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Breeding for durable Striga resistance requires identifi cation and stacking of multiple genetic barriers to parasitism in crops. Th is task would be greatly aided by having screening tools for identifying in parent sources and breeding populations resistance traits that function at various stages in the parasitic association. In this study, we describe a root observation system called the sand packed titer plate assay (SPTPA) used to study early postattachment development of Striga hermonthica (Del.) Benth. on two maize (Zea mays L.) and two sorghum [Sorghum bicolor (L.) Moench] inbred lines selected for fi eld resistance and susceptibility to Striga. Th e resistant maize and sorghum lines had fewer Striga attachments and these showed delayed parasitic development, and higher mortality compared to those on susceptible cultivars. Th e SPTPA was useful in identifying specifi c resistance reactions to S. hermonthica in maize and sorghum and refl ected results from fi eld studies of these varieties. Th is assay would complement fi eld testing during development of Striga-resistant varieties.

Item Type: Article
Author Affiliation: Dep. of Agronomy, Lilly Hall of Life Sciences, 915 W. State St., Purdue Univ., West Lafayette, IN 47907-2054
Subjects: Crop Improvement > Plant Breeding
Divisions: Maize
Depositing User: Sandhya Gir
Date Deposited: 05 Sep 2011 09:52
Last Modified: 05 Sep 2011 09:52
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