In vitro multiplication and conservation of Saccharum germplasm

Chandran, K. (2010) In vitro multiplication and conservation of Saccharum germplasm. Indian Journal of Plant Genetic Resources, 23 (1). pp. 65-68.

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Sugarcane germplasm have been maintained in the world germplasm repository at Sugarcane Breeding Institute Research Centre in the field gene bank. Field gene bank is always at the risk of biotic and abiotic factors and complimentary strategies need to be worked out to support the field gene bank. In vitro techniques offer different methods of conservation, and slow growth preservation is being successfully employed in many vegetative propagated crops. In this study, attempt was made to standardize optimal in vitro multiplication of different species of Saccharum and also studied to delay the sub-culturing period by retarding the growth of the in vitro-maintained material. It was observed that MS medium supplemented with 1 mg/l Kn and 6 mg/l BAP was more effective in inducing multiple shoots from the shoot apex in most of the species studied and 2% mannitol was found effective in controlling the growth.

Item Type: Article
Author Affiliation: Sugarcane Breeding Institute Research Centre, Kannur, Tamil Nadu
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Plant Physiology and Biochemistry > Plant Physiology
Plant Physiology and Biochemistry > Biochemistry
Divisions: Other Crops
Depositing User: Sandhya Gir
Date Deposited: 25 Oct 2010 03:39
Last Modified: 25 Oct 2010 03:39
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