Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.)

Zavallo, D and Bilbao, M L and Hopp, H E and Heinz, R (2010) Isolation and functional characterization of two novel seed-specific promoters from sunflower (Helianthus annuus L.). Plant Cell Reports, 29 (3). pp. 239-248.

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The promoter region of two sunflower (Helianthus annuus L. HA89 genotype) seed specifically expressed genes, coding for an oleate desaturase (HaFAD2-1) and a lipid transfer protein (HaAP10), were cloned and in silico characterized. The isolated fragments are 867 and 964 bp long, respectively, and contain several seed-specific motifs, such as AACA motif, ACGT element, E-Boxes, SEF binding sites and GCN4 motif. Functional analysis of these promoters in transgenic Arabidopsis plants was investigated after fusing them with the β-glucuronidase (GUS) reporter gene. None of the promoters triggered GUS activity in any vegetative tissue, with the exception of early seedling cotyledons. HaFAD2-1 and HaAP10 promoters were tested along seed development from globular stage to mature seeds. GUS staining was restricted to embryonic tissue and quantitative fluorometric assays showed high activity values at the later stages of development. In this work we demonstrate that HaFAD2-1 promoter is as strong as 35S promoter even though it is a tissue-specific promoter and its activity derived just from the embryo, thus confirming that it can be considered a strong highly specific seed promoter useful for biotechnology applications. Electronic supplementary material The online version of this article (doi:10.1007/s00299-010-0816-x) contains supplementary material, which is available to authorized users.

Item Type: Article
Uncontrolled Keywords: Seed promoter - Sunflower - Oleate desaturase - Lipid transfer protein
Author Affiliation: Instituto de Biotecnología, CNIA, Instituto Nacional de Tecnología Agropecuaria (INTA)-Castelar, Los Reseros y Nicolas Repeto
Subjects: Plant Production
Plant Protection
Statistics and Experimentation > Experimentation
Depositing User: Mr. SanatKumar Behera
Date Deposited: 06 Jan 2012 09:57
Last Modified: 06 Jan 2012 09:57
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