Development of genic molecular markers linked to a rust resistance gene in cultivated groundnut (Arachis hypogaea L.)

Mondal, S. and Badigannavar, A.M. and D’Souza, S.F. (2012) Development of genic molecular markers linked to a rust resistance gene in cultivated groundnut (Arachis hypogaea L.). Euphytica. pp. 1-11.

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Development of molecular markers for different economically important traits in cultivated groundnut has progressed at slow pace. Although many genomic SSR markers were developed in both the wild and cultivated groundnut, the genetic linkage map in the species is still not saturated. Availability of a large number of ESTs in GenBank opened up the possibility of integrating new markers and to identify markers closely linked to agronomic traits. EST-SSR markers are also considered as genic molecular markers. In this study, 259 EST-SSR markers were developed by mining 5,184 Arachis hypogaea ESTs from NCBI database. These EST-SSRs and 34 resistance gene candidate markers were used for association and genetic mapping of rust resistance in cultivated groundnut. From these, Cer2, SSR_GO340445, SSR_HO115759, SSR_GO341324 and RGC 2 had a significant association with rust resistance based on locus-by-locus AMOVA and/or Kruskal–Wallis ANOVA. Some of these associated markers also had protein activity related to biotic stress responses. Through genetic mapping, EST-SSR markers SSR_GO340445 and SSR_HO115759 were found closely linked to a rust resistance gene at 1.9 and 3.8 cM distances, respectively. These markers are thus suitable candidates for marker assisted selection in groundnut. The tight linkage of SSR_GO340445 would be helpful to screen BAC clones and to isolate rust resistance gene in groundnut.

Item Type: Article
Additional Information: The authors are thankful to International Atomic Energy Agency for partial financial support (IND 12818). The technical help rendered by Shakthi, Anantha, R. K. Sachan and T. Chalapathi during laboratory and field experimentations is duly acknowledged.
Uncontrolled Keywords: Puccinia arachidis, EST-SSR, Rust resistance gene, RGC marker, Genetic linkage, Association mapping
Author Affiliation: Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Mumbai 400085, India
Subjects: Crop Improvement > Genetics/Genomics
Divisions: Groundnut
Depositing User: Mr Siva Shankar
Date Deposited: 20 Jan 2012 08:43
Last Modified: 20 Jan 2012 08:52
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