Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding

Huang, X.Q. and Hsam, S.L.K. and Zeller, F.J. and Wenzel, G. and Mohler, V. (2000) Molecular mapping of the wheat powdery mildew resistance gene Pm24 and marker validation for molecular breeding. Theoretical and Applied Genetics, 101 (3). pp. 407-414.

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Molecular markers were identified in common wheat for the Pm24 locus conferring resistance to different isolates of the powdery mildew pathogen, Erysiphe graminis DM f. sp. tritici (Em. Marchal). Bulked segregant analysis was used to identify amplified fragment length polymorphism (AFLP) markers and microsatellite markers linked to the gene Pm24 in an F2 progeny from the cross Chinese Spring (susceptible)× Chiyacao (resistant). Two AFLP markers XACA/CTA-407 and XACA/CCG-420, and three microsatellite markers Xgwm106, Xgwm337 and Xgwm458, were mapped in coupling phase to the Pm24 locus. The AFLP marker locus XACA/CTA-407 co-segregated with the Pm24 gene, and XACA/CCG-420 mapped 4.5 cM from this gene. Another AFLP marker locus XAAT/CCA-346 co- segregated in repulsion phase with the Pm24 locus. Pm24 was mapped close to the centromere on the short arm of chromosome 1D, contrary to the previously reported location on chromosome 6D. Pm24 segregated independently of gene Pm22, also located on chromosome 1D. An allele of microsatellite locus Xgwm337 located 2.4±1.2 cM from Pm24 was shown to be diagnostic and therefore potentially useful for pyramiding two or more genes for powdery mildew resistance in a single genotype.

Item Type: Article
Additional Information: The authors thank Dr. X.F. Wang for providing the wheat landrace, Dr. M. Röder for making microsatellite primer sequences publicly available and Carmen Möller for technical assistance. Financial support by Deutscher Akademischer Austauschdienst (DAAD) is gratefully acknowledged.
Uncontrolled Keywords: Bulked segregant analysis · Marker-assisted selection · Microsatellites · Powdery mildew resistance · Triticum aestivum
Author Affiliation: Technische Universität München(Germany)
Subjects: Plant Protection
Crop Improvement
Divisions: Other Crops
Depositing User: Mr Siva Shankar
Date Deposited: 10 Feb 2012 04:12
Last Modified: 10 Feb 2012 04:12
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