Dissecting the genome of the polyploid crop oilseed rape by transcriptome sequencing

Bancroft, I. and Morgan, C. and Fraser, F. (2011) Dissecting the genome of the polyploid crop oilseed rape by transcriptome sequencing. Nature Biotechnology, 29 (8). pp. 762-766.

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Polyploidy complicates genomics-based breeding of many crops, including wheat, potato, cotton, oat and sugarcane. To address this challenge, we sequenced leaf transcriptomes across a mapping population of the polyploid crop oilseed rape (Brassica napus) and representative ancestors of the parents of the population. Analysis of sequence variation1 and transcript abundance enabled us to construct twin single nucleotide polymorphism linkage maps of B. napus, comprising 23,037 markers. We used these to align the B. napus genome with that of a related species, Arabidopsis thaliana, and to genome sequence assemblies of its progenitor species, Brassica rapa and Brassica oleracea. We also developed methods to detect genome rearrangements and track inheritance of genomic segments, including the outcome of an interspecific cross. By revealing the genetic consequences of breeding, cost-effective, high-resolution dissection of crop genomes by transcriptome sequencing will increase the efficiency of predictive breeding even in the absence of a complete genome sequence

Item Type: Article
Author Affiliation: John Innes Centre, Norwich Research Park, Norwich, Norfolk, UK
Subjects: Crop Improvement > Genetics/Genomics
Divisions: Other Crops
Depositing User: Ms K Syamalamba
Date Deposited: 14 Feb 2012 05:43
Last Modified: 14 Feb 2012 05:43
Official URL: http://dx.doi.org/10.1038/nbt.1926
URI: http://eprints.icrisat.ac.in/id/eprint/3046

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