Phytochrome control of its own synthesis in Sorghum vulgare and Avena sativa

Otto, v and Mousinger, E and Sauter, M and Schafer, E (1983) Phytochrome control of its own synthesis in Sorghum vulgare and Avena sativa. Photochemistry and photobiology, 38 (6). pp. 693-700.

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The accumulation of phytochrome in the dark was measured for Avena sativa seedlings after a white light pretreatment and for Sorghum vulgare seedlings after continuous red or far-red light treatments, using the herbicide Norflurazon to prevent greening under continuous irradiation. In both cases the accumulation of phytochrome depends on the state of the phytochrome at the light-dark transition: high Pfr levels (red light pulse) led to a slower rate of phytochrome accumulation than lower Pfr levels (long wavelength far-red (RG 9) light pulse). Poly-(A+)-RNA was isolated fromA. sativa seedlings grown for 48 h in darkness + 24 h WL + light pulse (5 min) (red, RG 9 light, red followed by RG 9 light or RG 9 followed by red light pulse) + 19 h darkness. The poly-(A+)-RNA was translated in a rabbit reticulocyte lysate system and the translation products were immunoprecipitated by specific anti-phytochrome antibodies. It was demonstrated that the activity of mRNA coding for phytochrome was under phytochrome control.

Item Type: Article
Author Affiliation: Biological Institute II, University of Freiburg, Schanzlestrasse 1, D-7800 Freiburg, W.Germany.
Subjects: Plant Production > Farming Systems
Agricultural Engineering > Mechanization
Divisions: Sorghum
Depositing User: Library ICRISAT-InfoSAT
Date Deposited: 17 Mar 2012 10:03
Last Modified: 17 Mar 2012 10:03
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