Characterization of Groundnut bud necrosis virus infecting French bean

Akram, Md. and Naimuddin, - (2012) Characterization of Groundnut bud necrosis virus infecting French bean. Journal of Food Legumes, 25 (1). pp. 54-57.

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During rabi 2008–09 and 2009–10, a virus disease of French bean was characterized by chlorotic spots with irregular necrotic or non-necrotic margins and twisting of leaf lamina. Plants infected at early stage remained stunted. Disease incidence was recorded in the range of 4–5%. The causal virus was successfully sap transmitted from field infected plants to healthy plants of French bean and to cowpea cv. ‘Pusa Komal’, a diagnostic host for the Tospoviruses. Further, the identity of the virus was confirmed by reverse transcription-polymerase chain reaction using primer pairs (GBNV-MF 5’ ATG TCT CGC TTG TCT AAC GTC 3’/GBNV-MR 5’ TTA TAT TTC AAG AAG ATT AT C 3’) designed to target the NSm gene of the Groundnut bud necrosis virus (GBNV). Complete NSm gene of French bean isolate of GBNV was 924 nucleotides long, encoding a protein of 307 amino acids. Sequence analysis of the NSm gene revealed that the French bean isolate under study (HQ259244) shared maximum identity at nucleotide (98%) and amino acids (99%) levels with GBNV-[Mb] isolate (AY871097). With other GBNV isolates viz., type isolate (U42555) and cowpea isolate (AY221023), present isolate had respectively, 95 and 93% identity at nucleotide level and 99% similarity at amino acids level. The phylogenetic relationship analysis revealed that the present isolate of GBNV clustered with known isolates of GBNV. Thus, the virus causing chlorotic spot disease in French bean at Kanpur was identified as an isolate of GBNV and has been designated as GBNV-[Raj]. Full sequence of the NSm gene of a GBNV isolate infecting Phaseolus vulgaris is being reported here for the first time.

Item Type: Article
Uncontrolled Keywords: French bean, Groundnut bud necrosis virus, NSm gene, Phaseolu svulgaris.
Author Affiliation: Division of Crop Protection, Indian Institute of Pulses Research, Kanpur-208024, India
Subjects: Plant Protection
Divisions: Groundnut
Depositing User: Mr. SanatKumar Behera
Date Deposited: 24 Mar 2012 09:23
Last Modified: 24 Mar 2012 09:24
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