Separation of phenolics of sorghum bicolor (L.) Moench grain

Kaluza, W.Z. and McGrath, R.M. and Roberts, T.C. and Schroder, H.H. (1980) Separation of phenolics of sorghum bicolor (L.) Moench grain. Journal of Agricultural and Food Chemistry, 28 (6). pp. 1191-1196.

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Two column supports, useful in the resolution of sorghum grain phenolics, are introduced. The 1st, Sepharose CL-6B, divided the phenolics into 3 main groups, F1, F2, and F3, while the 2nd, Sephacryl S-200, subdivided the 3 fractions obtained from the Sepharose column. A continuous automated monitoring system is described for polyphenol detection. The system utilized either Folin-Ciocalteu reagent or a sol. of FeCl3 in Rochelle salt. On addition of alkali, the Folin detection system monitored the total phenolic hydroxyls present, while the FeCl3 method monitored only those hydroxyl phenolics which were spatially acceptable for complexing with the Fe3+. The F3 group phenolics were the largest in size and consisted of 2 polymeric forms, one of mol. wt. approx. 10 000 and the other of mol. wt. > 80000

Item Type: Article
Author Affiliation: National Food Res. Inst., CSIR, Pretoria 0001, South Africa
Subjects: Crop Improvement
Crop Improvement > Seed Technology
Divisions: Sorghum
Depositing User: Ms K Syamalamba
Date Deposited: 28 Mar 2012 05:55
Last Modified: 28 Mar 2012 05:55
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