Discovery and Characterization of Polymorphic Simple Sequence Repeats (SSRs) in Peanut

Hopkins, M S and Casa, A M and Wang, T and et al, . (1999) Discovery and Characterization of Polymorphic Simple Sequence Repeats (SSRs) in Peanut. Crop Science, 39 (4). pp. 1243-1247.

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Cultivated peanut (Arachis hypogaea L), an important agronomic crop, exhibits a considerable amount of variability for morphological traits and for resistance to diseases and pests. In contrast, molecular marker assays have detected little variation at the nucleic acid level. Identification of molecular markers would be of great help to peanut breeders, geneticists, and taxonomists. The objectives of this work were to identify simple sequence repeat (SSR) markers in cultivated peanut and to test these markers for their ability to discriminate among accessions. Peanut total genomic DNA libraries were constructed and screened with 32P-labeled dinucleotide repeats, (GT)10 and (CT)10. DNA sequences were obtained from the SSR-containing clones and, when possible, primer pairs were designed on the basis of DNA sequences flanking the repeat motif. Primer pairs were tested in polymerase chain reaction (PCR) assays using a collection of 22 peanut DNAs, representing both cultivated peanut and wild species. In all, six SSR markers, five from the library screening procedure and one additional marker obtained from a search of publicly available DNA sequences, detected polymorphisms among the peanut DNAs. Discrimination power was high among the cultivated peanuts, with 17 unique genotypes represented among the 19 accessions tested. From two to 14 DNA fragments were amplified per SSR marker, and as a group, the six markers may amplify up to 10 putive SSR loci. The SSR markers identified in this study were more effective in detecting molecular variation in cultivated peanut than all other DNA-based markers evaluated to date.

Item Type: Article
Additional Information: The authors thank H.T. Stalker and C.C. Holbrook for critical review of an earlier version of this manuscript. We also thank the regional S-9 project for partial support of this research.
Author Affiliation: USDA-ARS, Plant Genetic Resources Conservation Unit, 1109 Experiment Street, Griffin, GA 30223
Subjects: Crop Improvement
Divisions: Groundnut
Depositing User: Mr Siva Shankar
Date Deposited: 28 Mar 2012 06:10
Last Modified: 28 Mar 2012 06:10
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