Ethanol production from steam-pretreated sweet sorghum bagasse with high substrate consistency enzymatic hydrolysis

Shen, F. and Hu, J. and Zhong, Y. (2012) Ethanol production from steam-pretreated sweet sorghum bagasse with high substrate consistency enzymatic hydrolysis. Biomass and Bioenergy. pp. 1-8.

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In this work, separate hydrolysis and fermentation (SHF) and simultaneous saccharification fermentation (SSF) with high substrate consistency (the mass fraction: 12%) were performed with the steam-pretreated sweet sorghum bagasse (SSB). Fermentation ability of four yeast strains and influences of residual solids after hydrolysis on fermentation were investigated in SHF. Meanwhile, influences of inorganic salts on fermentation were assessed to determine their suitable supplementations for ethanol production. Additionally, influences of initial yeast inoculation on SHF and SSF were further investigated. The results showed the adopted yeast strain, Tembec 1, displayed the best fermentation performance on the hydrolysate of pretreated SSB, and the residual solids in hydrolysate had negligible influences on ethanol fermentation. Although the deficiency or overdose of (NH4)2HPO4 or MgSO4·7H2O could reduce ethanol yield in SHF, the suitable supplementation of (NH4)2HPO4 (0.5 g L−1) and MgSO4·7H2O (1.0 g L−1) could increase ethanol yield by 5.2% and 8.3%, respectively. The initial yeast inoculation of 3 g L−1 could satisfy both SSF and SHF, which achieved 63.8% and 57.9% ethanol yield of theoretical one with final ethanol concentration of 23.3 g L−1 and 21.2 g L−1, respectively. In addition, ethanol yield kept almost constant as yeast was inoculated from 3 to 5 g L−1 in SHF, whereas it decreased significantly in SSF

Item Type: Article
Uncontrolled Keywords: Sorghum bicolor (L.) Moench; Separate hydrolysis and fermentation; Simultaneous saccharification fermentation; High substrate consistency; Ethanol production
Author Affiliation: Provincial Key Laboratory of Agricultural Environmental Engineering, Sichuan Agricultural University, Chengdu Campus, 211 Huimin Road, Chengdu, Sichuan 611130, PR China
Subjects: Plant Production
Crop Improvement
Divisions: Sorghum
Depositing User: Mr. SanatKumar Behera
Date Deposited: 29 Mar 2012 05:43
Last Modified: 29 Mar 2012 05:43
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