Characterization of new microsatellite markers in mung bean, Vigna radiata (L.)

Gwag, J.G. and Chung, J.W. and Chung, H. and Lee, J.H. and Ma, K.H. and Dixit, A. and Park, Y.J. and Cho, E.G. and Kim, T.S. and Lee, S.H. (2006) Characterization of new microsatellite markers in mung bean, Vigna radiata (L.). Molecular Ecology Notes, 6 (4). pp. 1132-1134.

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The present work reports the isolation and characterization of new polymorphic microsatellites in mung bean (Vigna radiata L.). Of 93 designed primer pairs, seven were found to amplify polymorphic microsatellite loci, which were then characterized using 34 mung bean accessions. The number of alleles ranged from two to five alleles per locus with an average of three alleles. Observed and expected heterozygosity values ranged from 0 to 0.088 and from 0.275 to 0.683, respectively. All seven loci showed significant deviations from Hardy-Weinberg equilibrium, whereas only one pairwise combination (GBssr-MB77 and GBssr-MB91) exhibited significant departure from linkage disequilibrium. These newly developed markers are currently being utilized for diversity assessment within the mung bean germplasm collection of the Korean Gene Bank.

Item Type: Article
Author Affiliation: National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon 441-707, Korea Republic
Subjects: Crop Improvement
Crop Improvement > Plant Breeding
Divisions: Other Crops
Depositing User: Syamala
Date Deposited: 01 Nov 2010 22:57
Last Modified: 29 Dec 2010 20:52
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