Marker-assisted backcrossing for simultaneous introgression of two genes

Frisch, M. and Melchinger, A.E. (2001) Marker-assisted backcrossing for simultaneous introgression of two genes. Crop Science, 41 (6). pp. 1716-1725.

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Marker-assisted selection is used to accelerate recovery of the recurrent parent genome (RPG) in backcross programmes. Our objectives were to compare various selection strategies and breeding plans for the simultaneous introgression of two genes with respect to the RPG recovered and the number of marker data points (MDP) required. Computer simulations were performed with a published genetic map in maize consisting of 80 markers and assuming selection for dominant target genes on the basis of phenotypic evaluation. For unlinked as well as for linked target genes, a shortening of the backcross programme from six to three generations resulted from applying marker-assisted background selection. In breeding programmes with three backcross generations, the least MDP were required when (i) applying selection strategies consisting of three or four selection steps on the basis of presence of the target genes and selection indices calculated from the marker genotype, (ii) increasing the population size from early to advanced generations, and (iii) merging the target genes in an early generation. These principles can be used for optimizing the design of marker-assisted backcross programmes for the simultaneous introgression of two genes.

Item Type: Article
Author Affiliation: nstitute of Plant Breeding, Seed Science, and Population Genetics, University of Hohenheim, 70593 Stuttgart, Germany
Subjects: Crop Improvement > Plant Breeding
Divisions: General
Depositing User: Syamala
Date Deposited: 02 Nov 2010 15:32
Last Modified: 29 Dec 2010 20:52
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