A Staining Procedure to Determine the Extent of Bran Removal in Pearled Sorghum

Scheuring, J.F. and Rooney, L.W. (1979) A Staining Procedure to Determine the Extent of Bran Removal in Pearled Sorghum. Cereal Chemistry, 56 (6). pp. 545-548.

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The completeness of bran removal during milling is difficult to determine for many sorghum grains. Two dye systems that stain the germ, pericarp, and starchy endosperm of sorghum blue, green, and pink, respectively, are useful to determine the completeness of milling. The May-Grunwald solution (0.5% methylene blue and 0.5% eosin-Y in methanol) and o.25% methylene blue and 0.75% eosin-Y in 70% ethanol are equally effective. Samples of pearled kernels from each of three varieties milled by three processes were stained. The exposed starchy endosperm, pericarp fragments, and germ tissue stained pink, green, and blue, respectively. The staining procedures were especially useful for sorghums with a white pericarp. The methods are simple and can be efficiently used in milling studies and in breeding programs to select for sorghums with improved milling properties

Item Type: Article
Author Affiliation: Sorghum Breeding and Cereal Quality Lab, Soil and Crop Science Department, Texas A & M Unlversity, College Station TX 77843
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Ms K Syamalamba
Date Deposited: 30 Mar 2012 11:02
Last Modified: 30 Mar 2012 11:02
URI: http://eprints.icrisat.ac.in/id/eprint/4423

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