Resistance Allele Frequency to Bt Cotton in Field Populations of Helicoverpa armigera (Lepidoptera: Noctuidae) in China

Liu, F and Xu, Z and Chang, J and et al, . (2008) Resistance Allele Frequency to Bt Cotton in Field Populations of Helicoverpa armigera (Lepidoptera: Noctuidae) in China. Journal of Economic Entomology, 101 (3). pp. 933-943.

PDF - Published Version
| Preview


Resistance evolution in target insects to Bacillus thurningiensis (Bt) cotton, Gossypium hirsutum L., is a main threat to Bt cotton technology. An increasing trend of population density of Helicoverpa armigera (Hu¨ bner) (Lepidoptera: Noctuidae) has been observed since 2001 in Qiuxian County (Hebei, China), where Bt cotton has been planted dominantly since 1998. This region was selected in 2006 and 2007 for estimating frequency of gene alleles conferring resistance to Bt cotton by screening the F1 progeny from single-pair cross between Þeld-collected male and laboratory female of the Bt-resistant strain of H. armigera (F1 screen). F1 offspring from each single-pair line were screened for resistance alleles based on larval growth, development, and survival on Bt cotton leaves for 5 d. Two-year results indicated that �20% of Þeld-collected males carried resistance alleles. The conservative estimate of the resistance allele frequency was 0.094 (95% CI, 0.044Ð0.145) for 2006 and 0.107 (95% CI, 0.055Ð0.159) for 2007. This is the Þrst report of resistance allele frequency increase to such a high level in the Þeld in China. Long-term adoption of Bt sprays, dominant planting of single-toxinÐproducing Bt cotton, and lack of conventional cotton refuge system might accelerate the resistance evolution in the region.

Item Type: Article
Additional Information: We are grateful to Qiuxian County Plant Protection Station for providing facilities and Þeld data of bollworm occurrence. Special thanks to Fred Gould (North Carolina State University, Raleigh, NC) for valuable advices and the help with the data analysis and to Andrew Li (USDAÐARS, Kerrville, TX) and Lingxiao Zhang (DREC, Mississippi State University, Stoneville, MS) for valuable comments and suggestions for improving an earlier version of this manuscript. This research was supported by the Special Funding of Transgenic Plant Study and Its Industrialization Opening up and Developing (J00-C-002) and National ScientiÞc Research Fund (30270889).
Uncontrolled Keywords: Helicoverpa armigera, Bt cotton, resistance detection, F1 screen, resistance management
Author Affiliation: Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing, 210095, China.
Subjects: Plant Protection
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 16 Apr 2012 04:38
Last Modified: 16 Apr 2012 04:38

Actions (login required)

View Item View Item