Novel application of aniline blue

Shennawy, I E and Gee, D J and Horobin, R W and et al, . (1984) Novel application of aniline blue. Histochemistry and Cell Biology, 81 (1). pp. 93-98.

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The present study shows that aniline blue can be used as a fluorescent stain for glycogen. The dye is also helpful in tracing pathological and autolytic changes in lysosomes, mitochondria, erythrocytes and nuclei, and it can also be used for demonstrating bacteria in tissue sections and smears. The techniques used are simple, rapid and inexpensive. Spectrophotometric studies on aniline blue solutions have shown that aniline blue fluorescence was enhanced by the addition of certain proteins, or of glycogen to the dye solution. In case of albumen which has the maximum effect, enhancement is dependent upon the albumen-dye ratio. The mechanism of staining is mainly due to self quenching, but there is also an evidence of the presence of hydrophobic reaction.

Item Type: Article
Additional Information: We wish to thank Professor A.G.E. Pearse, Royal Postgraduate Medical School, University of London for his helpful advice. We also thank Dr. A. Ghoniem, Microbiology Department, St. James' Hospital, Leeds and Dr. J.B.C. Findlay, Biochemistry Department, University of Leeds for the helpful discussions. We are indebted to Mr. P. Lawrence, Forensic Medicine Department, Leeds for his assistance during preparation of erythrocyte ghosts and mitochondrial pellets. Finally we thank Mr. P. Gaunt and Miss P. Gaunt for expert technical assistance and Mrs. J. Rodger and Miss P. Whitaker for the excellent secretarial aid and typing the manuscript.
Author Affiliation: Department of Forensic Medicine and Pathology, Clinical Sciences Building, St. James's University Hospital, Beckett Street, Leeds LS9 7TF, UK
Subjects: Crop Improvement > Genetics/Genomics
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 16 Apr 2012 09:31
Last Modified: 16 Apr 2012 09:32
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