Using an F2 screen to monitor frequency of resistance alleles to Bt cotton in field populations of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae)

Xu, Z and Liu, F and Chen, J and et al, . (2009) Using an F2 screen to monitor frequency of resistance alleles to Bt cotton in field populations of Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae). Pest Management Science, 65 (4). pp. 391-397.

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BACKGROUND: In an effort to control the most devastating cotton pest, Helicoverpa armigera (H ¨ ubner), in Qiuxian County (Hebei, China), Bt cotton has been planted almost exclusively since 1998 in this area. Owing to the high insecticidal selection pressures in this region, monitoring of Bt resistance in H. armigera is necessary so that proactive actions can be implemented before field control measures fail. From 2003 to 2005, an F2 screen was conducted in order to monitor Bt resistance in H.armigera populations collected from this area. RESULTS: The F2 screen showed that 15 out of 278 isofemale lines carried resistance alleles to Bt cotton. The resistance allele frequency in field populations of H. armigera ranged from 0.0119 to 0.0297, with an overall frequency of 0.0146 and a 95% confidence interval of 0.0084–0.0225 for the 3 year period. This value is greater than the value reported from 1999 (P < 0.05). CONCLUSION: A fluctuating but overall increase in resistance allele frequency was detected in the field populations of H. armigera in Qiuxian County from 1999 to 2005. To prevent further increases in Bt resistance frequency in this pest, it is necessary to introduce Bt cotton expressing multiple Bt toxins and integrate this technology with other tactics for management of this key pest.

Item Type: Article
Additional Information: The authors are grateful toQiuxianCounty Plant Protection Station for providing facilities and field data of bollworm occurrence. Special thanks are due to Dr Xueming Liu (Kansas State University, Manhattan, KS) and Dr Huarong Li (Iowa State University, Ames, IA) for their valuable comments and suggestions for improving an early version of this manuscript. This research was supported by Special Funding of Transgenic Plant Study and Industrialization Opening-up and Development (J00-C-002) and the National Scientific Research Fund (30270889).
Uncontrolled Keywords: Helicoverpa armigera; F2 screen; Bt cotton; Bacillus thuringiensis; resistance; allele
Author Affiliation: Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China
Subjects: Plant Protection
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 20 Apr 2012 03:40
Last Modified: 20 Apr 2012 03:40
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