Multiparent intercross populations in analysis of quantitative traits

Rakshit, S and Rakshit, A and Patil, J V (2012) Multiparent intercross populations in analysis of quantitative traits. Journal of Genetics, 91 (1). pp. 1-7.

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Most traits of interest to medical, agricultural and animal scientists show continuous variation and complex mode of inheritance. DNA-based markers are being deployed to analyse such complex traits, that are known as quantitative trait loci (QTL). In conventional QTL analysis, F2, backcross populations, recombinant inbred lines, backcross inbred lines and double haploids from biparental crosses are commonly used. Introgression lines and near isogenic lines are also being used for QTL analysis. However, such populations have major limitations like predominantly relying on the recombination events taking place in the F1 generation and mapping of only the allelic pairs present in the two parents. The second generation mapping resources like association mapping, nested association mapping and multiparent intercross populations potentially address the major limitations of available mapping resources. The potential of multiparent intercross populations in gene mapping has been discussed here. In such populations both linkage and association analysis can be conductted without encountering the limitations of structured populations. In such populations, larger genetic variation in the germplasm is accessed and various allelic and cytoplasmic interactions are assessed. For all practical purposes, across crop species, use of eight founders and a fixed population of 1000 individuals are most appropriate. Limitations with multiparent intercross populations are that they require longer time and more resource to be generated and they are likely to show extensive segregation for developmental traits, limiting their use in the analysis of complex traits. However, multiparent intercross population resources are likely to bring a paradigm shift towards QTL analysis in plant species.

Item Type: Article
Additional Information: The author thank Drs P. Rajendra Kumar and K. N. Ganapathy, Directorate of Sorghum Research for their valuable suggestions in improving the manuscript.
Uncontrolled Keywords: gene mapping; QTL analysis; multiparent intercross population; MAGIC; AMPRIL; association mapping.
Author Affiliation: Directorate of Sorghum Research, Rajendranagar, Hyderabad 500 030, India
Subjects: Crop Improvement
Divisions: Sorghum
Depositing User: Mr Siva Shankar
Date Deposited: 23 Apr 2012 03:54
Last Modified: 07 May 2012 05:28

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