Developmental and Environmental Regulation of a Bean Chalcone Synthase Promoter in Transgenic Tobacco

Schmid, J. and Doerner, P. W. and Clouse, S. D. and et al, . (1990) Developmental and Environmental Regulation of a Bean Chalcone Synthase Promoter in Transgenic Tobacco. The Plant Cell, 2 (7). pp. 619-631.

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Regulatory properties of a 1.4-kilobase promoter fragment of the bean chalcone synthase CHS8 gene were examined by analysis of glucuronidase (GUS) activity in transgenic tobacco containing a CHS8-GUS gene fusion. The promoter was highly active in the root apical meristem and in petals, exclusively in those cells of the inner epidermis that accumulate anthocyanins. The gene fusion was only weakly expressed in other floral organs, mature leaves, and stems. The early stages of seedling development were characterized by an apparent wound induction of the promoter in the endosperm and strong expression in the immature root, which became localized to the apical meristem and perivascular tissue at the root-hypocotyl junction. The promoter became active during lateral root formation in both the new root and damaged tissue of the main root. The gene fusion was also expressed in greening cotyledons and primary leaves but not in the shoot apical meristem. Light modulated expression in the cotyledons and root-shoot junction but had no effect on other aspects of the developmental program. Wounding or funga1 elicitor treatment of mature leaves activated the promoter in a well-defined zone adjacent to the stress site. Stress induction occurred in mesophyll and vascular tissues as well as in the epidermis. We conclude that the CHS8 promoter contains cis-elements required to establish temporal and spatial control of flavonoid biosynthesis during development and in response to diverse environmental stimuli.

Item Type: Article
Author Affiliation: Plant Biology Laboratory, Salk lnstitute for Biological Studies, 1 O01 O North Torrey Pines Road, La Jolla, California 92037
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Mr Siva Shankar
Date Deposited: 27 Apr 2012 08:23
Last Modified: 27 Apr 2012 08:23
Official URL:

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