Comparative study of constitutive and inducible promoters in tobacco

Kálai, K. and Mészáros, A. and Dénes, F. and Balázs, E. (2008) Comparative study of constitutive and inducible promoters in tobacco. South African Journal of Botany, 74. pp. 313-319.

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The 42 kDa endochitinase gene of Trichoderma hamatum was expressed in tobacco, led by the constitutive 35S promoter of Cauliflower Mosaic Virus and the inducible Actin 7 promoter of Arabidopsis thaliana. Transgenic tobacco events containing the 35S promoter had increased chitinase levels in the leaves and enhanced tolerance to Botrytis cinerea, but their stems were still susceptible to infection. When the Actin 7 promoter was used, the resulting transgenic tobacco events exhibited tolerance to the pathogen even in the stem. The presence of the transgene was monitored by PCR analysis and the sequence of the PCR product confirmed the integration of the chitinase gene. Analysis of the transgenic plants showed that the lines were heterogeneous. Southern hybridization confirmed a single copy of the transgene in lines transformed with the 35S promoter, while the copy number was 1–4 in lines transformed with the Actin 7 promoter. Compared to the untransformed control, chitinase activity was higher in the leaves of the transgenic plants. This increase was not observed in the stems. In this comparative promoter analysis the stress-induced promoter proved to be more effective in obtaining mould-resistant plants.

Item Type: Article
Additional Information: This work was supported by the Ministry of Agriculture and Rural Development (FVM 46011). Thanks are due to Dr. Brigitta Dudás, Dr. László Palkovics, Dr. Barnabás Jenes and Endre Sebestyén for their contributions, to Márta Csányi and Boglárka Hajdú for their technical help, to Mrs Barbara Harasztos for her editorial help in preparing the manuscript, to Professor Richard Meagher, University of Georgia, for providing the Actin 7 promoter and to Professor László Hornok, Szent István University, Hungary for providing the T. hamatum endochitinase gene.
Uncontrolled Keywords: Actin 7 promoter; CaMV 35S promoter; Chitinase; Mould resistance; Transformation
Author Affiliation: Department of Plant Pathology, Faculty of Horticultural Sciences, Corvinus University of Budapest, Ménesi út 44., H-1118 Budapest, Hungary
Subjects: Crop Improvement
Divisions: Other Crops
Depositing User: Mr Siva Shankar
Date Deposited: 27 Apr 2012 08:26
Last Modified: 27 Apr 2012 08:26

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