Testing of a heterologous, wound- and insect-inducible promoter for functional genomics studies in conifer defense

Godard, Kimberley-Ann and Byun-McKay, A. and Levasseur, C. and et al, . (2007) Testing of a heterologous, wound- and insect-inducible promoter for functional genomics studies in conifer defense. Plant Cell Reports, 26 (12). pp. 2083-2090.

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Large-scale sequencing of conifer cDNAs and targeted molecular cloning have identified many putative conifer defense genes. Expression of many of these genes is induced in response to biotic stress and some may be expressed only in a few specialized tissues or cells. Proven functional genomics approaches to test these genes involve expression of proteins in Escherichia coli or yeast for biochemical characterization or constitutive over-expression in transformed plants. Plant transformation to test functions of insect-, wound- or pathogen-induced conifer defense genes would benefit from the use of an inducible expression system. We describe here the development of a heterologous, wound- and insect-inducible gene expression system for conifers using the potato proteinase inhibitor II (pinII)-promoter. Using pinII::GUS and pinII::(E)-abisabolene synthase expression constructs we demonstrate localized induced gene expression in white spruce seedlings (Picea glauca). Testing of these constructs in Arabidopsis thaliana and tobacco illustrates the additional potential of the pinII-promoter to be used in tests of gene function that involve cell-specific and systemic induction.

Item Type: Article
Additional Information: The research reported in this paper was supported with grants from the Natural Sciences and Engineering Research Council of Canada (NSERC; Jo¨rg Bohlmann and Aine Plant) and by funds from Genome British Columbia and Genome Canada in support of the TREENOMIX Conifer Forest Health Project (grant to Jo¨rg Bohlmann). Conifer transformation in the laboratory of Armand Se´guin was supported by the Arborea Project funded by Genome Que´bec and Genome Canada. We thank Dr. Rodney Croteau, Washington State University, Pullman, USA for providing the grand fir (E)-a-bisabolene synthase cDNA clone. We thank Gholamreza Babajani, Harpreet Sandhu and Franc¸oise Pelletier for technical assistance. Kimberley-Ann Godard was supported by an NSERC graduate student scholarship. Jo¨rg Bohlmann is an NSERC E.W.R. Steacie Memorial fellow.
Uncontrolled Keywords: Local and systemic defense, Metabolic, engineering, Oleoresin, Plant–insect interactions, Secondary metabolism, Terpenoid synthase
Author Affiliation: Michael Smith Laboratories, University of British Columbia, 321-2185 East Mall, Vancouver, BC, Canada V6T 1Z4
Subjects: Crop Improvement > Genetics/Genomics
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 27 Apr 2012 08:31
Last Modified: 27 Apr 2012 08:31
Official URL: http://dx.doi.org/10.1007/s00299-007-0417-5
URI: http://eprints.icrisat.ac.in/id/eprint/4989

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