Molecular characterization of an atoxigenic Aspergillus flavus strain AF051

Jiang, J. and Yan, L. and Ma, Z. (2009) Molecular characterization of an atoxigenic Aspergillus flavus strain AF051. Appl Microbiol Biotechnol, 83. pp. 501-505.

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An atoxigenic Aspergillus flavus strain AF051 collected from a peanut field in Jiangsu province, P. R. China was characterized by analysis of aflatoxin gene cluster in this study. By using a thermal asymmetric interlaced PCR (TAIL-PCR) and conventional PCR techniques, an 89.59-kb deletion was found in the cluster, and this deletion was replaced by a 3.83-kb insert, which was located at 300-bp upstream ver1 gene and 2594-bp downstream a putative gluconolactone oxidase gene. Based on the DNA sequence at the breakpoint, a nested-PCR method was developed for the rapid and sensitive detection of AF051 strain in soil and peanut samples once the strain is used as a biological agent.

Item Type: Article
Additional Information: This work was funded by a Project supported by the Science and Technology Program of Zhejiang Province of China (No. 2006C12087), the 863 program (2007AA10Z422) and the New Century Talent Program from Ministry of Education, China (No. NCET-06-0518) to Z. Ma.
Uncontrolled Keywords: Aspergillus flavus, Atoxigenic strain, Aflatoxin gene, cluster, Insert
Author Affiliation: Institute of Biotechnology, Zhejiang University, Hangzhou 310029, China
Subjects: Plant Protection
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 08 May 2012 05:29
Last Modified: 08 May 2012 05:29
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