Callus induction and regeneration of elite Indian maize inbreds

Manivannan, A. and Kaul, J. and Singode, A. and Dass, S. (2010) Callus induction and regeneration of elite Indian maize inbreds. African Journal of Biotechnology, 9 (44). pp. 7446-7452.

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Five elite Indian maize inbreds namely; HKI1105, HKI1105, HKI335, CM300 and LM5 were evaluated for callus induction and regeneration. Immature embryos obtained 14 days after pollination were used as explants. Genotype, medium, type of auxin and their concentrations influenced callus induction. N6 medium supplemented with different concentration of 2,4-D (1, 2 and 3 mg/l) and Dicamba (1, 2 and 3 mg/l) were used for callus induction. N6 supplemented with 2 mg/l of 2,4-D has shown highest percentage of embryogenic callus induction. Among the five genotypes tested, CM300 gave highest percentage of embryogenic calli. CM300 and LM5 both have shown higher regeneration percentage of 12.22%.

Item Type: Article
Author Affiliation: Directorate of Maize Research, Pusa Campus, New Delhi-110012, India.
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Biotechnology
Divisions: Maize
Depositing User: Mr T L Gautham
Date Deposited: 16 Nov 2010 21:01
Last Modified: 29 Dec 2010 20:40
Official URL:

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