Proteases in germinating finger millet (Eleusine coracana) seeds

Vidyavathi, U. and Shivaraj, B. and Pattabiraman, T.N. (1983) Proteases in germinating finger millet (Eleusine coracana) seeds. Journal of Bioscience, 5 (3). pp. 219-224.

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Proteolytic activity was estimated in germinated finger millet seedlings using the endogenous trypsin/amylase inhibitor as substrate and also with haemoglobin and albumin as substrates. The maximal proteolytic activity was observed on the third day of germination. With the inhibitor as substrate, the proteolytic activity was maximal at pH 2.5. The protease that acted on the inhibitor required sulphydryl groups for maximal activity and was suppressed by diazoacetyl norleucine methyl ester and Pepstatin. The protease that acted on haemoglobin with optimum pH of 5.0, was more stable on storage, did not depend on sulphydryl groups for activity and was unaffected by reagents that react with carboxyl groups.

Item Type: Article
Author Affiliation: Department of Biochemistry, Kasturba Medical College, Manipal
Subjects: Crop Improvement
Divisions: Millet
Depositing User: Mr. SanatKumar Behera
Date Deposited: 17 May 2012 06:27
Last Modified: 17 May 2012 06:27

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