Agrobacterium tumefaciens-mediated transformation of biofuel plant Jatropha curcas using kanamycin selection

Pan, Jingli and Fu, Qiantang and Xu, Zeng-Fu (2010) Agrobacterium tumefaciens-mediated transformation of biofuel plant Jatropha curcas using kanamycin selection. African Journal of Biotechnology , 9 (39). pp. 6477-6481.

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Abstract

Establishment of an efficient transformation system is a prerequisite for genetic improvement of Jatropha curcas, a promising biodiesel feedstock plant, by transgenic approach. In this study an efficient Agrobacterium-mediated transformation protocol using cotyledon explants from J. curcas seeds was developed. The integration and expression of the transgenes in the putatively transformed J. curcas plants was confirmed by polymerase chain reaction (PCR), Southern blot analysis and �- glucuronidase (GUS) activity staining. In the protocol used, the critical step for successful transformation is that the selective agent kanamycin (20 mg/L) is not included in the callus-inducing medium within the first 4 weeks after co-cultivation with Agrobacterium.

Item Type: Article
Author Affiliation: State Key Laboratory of Biocontrol and Key Laboratory of Gene Engineering of the Ministry of Education, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, Guangdong, China.
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Biotechnology
Divisions: Other Crops
Depositing User: Mr T L Gautham
Date Deposited: 16 Nov 2010 17:12
Last Modified: 29 Dec 2010 20:38
Official URL: http://www.academicjournals.org/AJB/PDF/pdf2010/27...
URI: http://eprints.icrisat.ac.in/id/eprint/552

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