Molecular relatedness within the “Ascochyta pinodes-complex”

Fatehi, J. and Bridge, P.D. and Punithalingam, E. (2003) Molecular relatedness within the “Ascochyta pinodes-complex”. Mycopathologia , 156 (4). pp. 317-327.

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The morphological, molecular and pathological characters of 34 isolates identified as Ascochyta pinodes, A. phaseolorum, and Phoma medicaginis var. pinodella were determined. Initial molecular findings, based on PCR fragment size and RFLPs of sections of the mitochondrial genome, the rRNA gene cluster and the -tubulin gene complex, were further investigated by sequencing of the total rRNA ITS regions. Isolates were homogenous for all characteristics determined, with the exception of conidial size and septation, and RFLPs of AT rich (presumptive mitochondrial) DNA. The results suggest that the isolates represent host preferential forms of a single taxon, and this finding is discussed in relation to the known teleomorphs and reference material of the species studied.

Item Type: Article
Uncontrolled Keywords: ITS sequences - mitochondrial DNA - molecular determination - Phoma
Author Affiliation: CABI Bioscience, Swedish University of Agricultural Sciences, University of London, ycology Section, The Herbarium, Royal Botanic Gardens
Subjects: Crop Improvement
Divisions: General
Depositing User: Mr. SanatKumar Behera
Date Deposited: 21 May 2012 06:38
Last Modified: 21 May 2012 06:38
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