A Modified Ninhydrin Colorimetric Analysis for Amino Acids

Rosen, H. (1957) A Modified Ninhydrin Colorimetric Analysis for Amino Acids. Archives of Biochemistry and Biophysisc, 67. pp. 10-15.

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In 1948, Stein and Moore published the first practical procedure for the chromatographic separation of amino acids on starch columns, and a photometric method for the quantitative analysis of the effluent fractions (1, 2). Their procedure was based on the reaction of amino acids with reduced ninhydrin (triketohydrindenhydrate) at pH 5; stannous chloride served as the reducing agent. The method is reliable and reproducible.

Item Type: Article
Additional Information: The work of Dr. Sterling Chaykin, who correlated much of the experimental data, is much appreciated. The advice and interest of Dr. Stanley M. Levenson are gratefully acknowledged.
Author Affiliation: Department of Surgical Metabolism, Division of Surgery, Walter Reed Army Institute of Research, Walter Reed Army Medical Center, Washington, D. C.
Subjects: Plant Physiology and Biochemistry > Biochemistry
Divisions: General
Depositing User: Mr Siva Shankar
Date Deposited: 29 May 2012 04:04
Last Modified: 29 May 2012 04:04
URI: http://eprints.icrisat.ac.in/id/eprint/5864

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