Marker-assisted Gene Pyramiding for Inbred Line Development: Basic Principles and Practical Guidelines

Ye, G. and Kevin, F.S. (2008) Marker-assisted Gene Pyramiding for Inbred Line Development: Basic Principles and Practical Guidelines. International Journal of Plant Breeding, 2 (1-2). pp. 1-10.

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Gene pyramiding, which aims to assemble multiple desirable genes into a single genotype, is a commonly used method in breeding for self-pollinated crops. Traditionally, the main use of gene pyramiding is to improve an existing elite cultivar through introgression of a few genes of large effects from other sources, since the presence of the target genes has to be monitored by phenotyping, which is only effective for major genes. Depending on the trait and inheritance of the targeted genes, gene pyramiding may require much labour, time and material resources. The development of modern plant molecular techniques and quantitative genetics in the last two decades has dramatically widened the applicability of gene pyramiding. It provides enhanced knowledge of the genetics of the breeding traits and of the relative genomic location of functionally related as well as neutral markers associated with the genes responsible for the traits. It facilitates the identification of genes with large effect for traits which are traditionally regarded as quantitative and not targeted by gene pyramiding program. Marker-based selection reduces/eliminates extensive phenotyping, provides more effective options to control linkage drag, makes the pyramiding of genes with very similar phenotypic effects possible, and reduces the breeding duration. Marker-based gene pyramiding is now the method of choice for inbred line development targeted at improving traits controlled by major genes. In this review, we focus on aspects of designing an efficient marker-based gene pyramiding strategy for inbred line development. The basic principles of gene pyramiding, the process and useful guidelines for designing an efficient strategy, and the integration of gene discovery and pyramiding are discussed in this paper, while the successful use of gene pyramiding in practical breeding is summarised in a companion paper

Item Type: Article
Subjects: Crop Improvement > Plant Breeding
Divisions: General
Depositing User: Syamala
Date Deposited: 15 Nov 2010 16:09
Last Modified: 29 Dec 2010 20:33
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