X-ray contrast method for seed viability determination of cereals and millets

Verma, M.M. and Verma, K. and Singh, A. (1975) X-ray contrast method for seed viability determination of cereals and millets. Seed Science and Technology, 3 (3-4). pp. 797-802.

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In an attempt to standardize the X-ray contrast method, various cereal and millet seeds were subjected to different soaking and 10 or 20% BaCl2 treatments. A 2-h treatment of the presoaked seeds of wheat, jowar (Sorghum bicolor) and pearl millet (Pennisetum americanum) with 20% BaCl2 and a 4-h treatment of maize seed, also with 20% BaCl2, gave the best contrast between impregnated (dead) and unimpregnated (viable) seeds. Other smaller millets (Eleusine coracana and Paspalum scrobiculatum) required treatment for 4 h after presoaking for 16 h, and in oats barley and rice a clear impregnation was not obtained. Alternative contrast agents were evaluated and it is suggested that the contrast is emphasized by slight over-developing of X-ray plates or treatment with lactophenol after BaCl2 treatment

Item Type: Article
Author Affiliation: Division of Seed Technology, Indian Agricultural Research Institute, New Delhi, India
Subjects: Crop Improvement > Seed Technology
Divisions: Millet
Depositing User: Ms K Syamalamba
Date Deposited: 12 Jul 2012 06:03
Last Modified: 12 Jul 2012 06:03
URI: http://eprints.icrisat.ac.in/id/eprint/6709

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