Genome wide analysis of disease resistance mlo gene family in sorghum [sorghum bicolor (l.) Moench]

Singh, V.K. and Singh, A.K. and Chand, R. and Singh, B.D. (2012) Genome wide analysis of disease resistance mlo gene family in sorghum [sorghum bicolor (l.) Moench]. Journal of Plant Genomics, 2 (1). pp. 18-27.

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Powdery mildew of sorghum (Erysiphe pisi var. pisi) is of worldwide occurrence and causes substantial yield losses. As far as resistance against powdery mildew is concerned, the presence of powdery mildew-resistance gene O (Mlo) plays a key role. The Mlo gene was first identified in barley, and the Mlo protein was found to be an integral plasma membrane-localized protein that possesses seven transmembrane regions. In the present work a total of 12 well reported Mlo genes from Oryza sativa, 15 well characterized Mlo genes from Arabidopsis thaliana genome were taken for comparative studies in sorghum (Sorghum bicolor). Comparative analysis of Mlo proteins from S. bicolor genome revealed the presence of 13 hypothetical genes within the genome. Map viewer analysis indicates that the predicted S. bicolor SbMlo genes are distributed on eight of the ten chromosomes. Sorghum chromosome 9 has 3 genes; chromosome 10 and chromosome 1 have 2 genes each, while chromosomes 2, 3, 4 and 5 have 1 gene each. Sub-cellular localization of identified Mlo proteins encoded by genes SbMlo1, SbMlo2, SbMlo4, SbMlo5, SbMlo6, SbMlo9, SbMlo10, SbMlo11, SbMlo12 and SbMlo13 are present in plasm membrane; SbMlo3 product is located in endoplasmic reticulum, SbMlo7encoded protein is located in vacuolar membrane and SbMlo8 product is present in the nuclear region. In silico characterization (using phylogenetic classification, motif analysis and cis-acting elements studies) suggested its diverse function associated with disease resistance based on specific expression containing fungal elicitor responsive elements. Gene specific primers, expression primers and universal primer were designed to check the expression and availability of SbMlo genes through wet lab experimentation.

Item Type: Article
Author Affiliation: Banaras Hindu University, Varanasi, India
Subjects: Plant Protection
Crop Improvement
Divisions: Sorghum
Depositing User: Mr. SanatKumar Behera
Date Deposited: 07 Aug 2012 10:04
Last Modified: 07 Aug 2012 10:06
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