Determination of cyclopiazonic acid in peanuts and corn by thin layer chromatography

Lansden, J.A. (1986) Determination of cyclopiazonic acid in peanuts and corn by thin layer chromatography. Journal of the Association of Official Analytical Chemists , 69 (6). pp. 964-966.

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A thin layer chromatographic system including densitometry has been developed for determining cyclopiazonic acid in peanuts and corn. Samples are extracted with methanol-chloroform (20 + 80); the extract is stripped of most interferences by partitioning with aqueous sodium bicarbonate followed by acidification and repartitioning with chloroform. After thin layer chromatography and derivatization with dimethylaminobenzaldehyde-HCl spray, the toxin is quantitated by reflection densitometry at 540 nm. The recovery of cyclopiazonic acid averages 90% for peanuts and 85% for corn. The absolute detection limit is 25 ng per spot which translates to a detection limit of 125 micrograms/kg for a 50 g sample.

Item Type: Article
Author Affiliation: U.S. Department of Agriculture, Agricultural Research Service, National Peanut Research Laboratory
Subjects: Statistics and Experimentation > Experimentation
Crop Improvement
Plant Physiology and Biochemistry > Biochemistry
Divisions: Groundnut
Other Crops
Depositing User: Mr. SanatKumar Behera
Date Deposited: 08 Aug 2012 05:15
Last Modified: 08 Aug 2012 05:19

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