Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake

Vila-Aiub, M.M. and Balbi, M.C. and Distéfano, A.J. and Fernández, L. and Hopp, E. and Yu, Q. and Powles, S.B. (2012) Glyphosate resistance in perennial Sorghum halepense (Johnsongrass), endowed by reduced glyphosate translocation and leaf uptake. Pest Management Science, 68 (3). pp. 430-436.

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BACKGROUND: In a large cropping area of northern Argentina, Sorghum halepense (Johnsongrass) has evolved towards glyphosate resistance. This study aimed to determine the molecular and biochemical basis conferring glyphosate resistance in this species. Experiments were conducted to assess target EPSPS gene sequences and 14C-glyphosate leaf absorption and translocation to meristematic tissues. RESULTS: Individuals of all resistant (R) accessions exhibited significantly less glyphosate translocation to root (11% versus 29%) and stem (9% versus 26%) meristems when compared with susceptible (S) plants. A notably higher proportion of the applied glyphosate remained in the treated leaves of R plants (63%) than in the treated leaves of S plants (27%). In addition, individuals of S. halepense accession R2 consistently showed lower glyphosate absorption rates in both adaxial (10–20%) and abaxial (20–25%) leaf surfaces compared with S plants. No glyphosate resistance endowing mutations in the EPSPS gene at Pro-101–106 residues were found in any of the evaluated R accessions. CONCLUSION: The results of the present investigation indicate that reduced glyphosate translocation to meristems is the primary mechanism endowing glyphosate resistance in S. halepense from cropping fields in Argentina. To a lesser extent, reduced glyphosate leaf uptake has also been shown to be involved in glyphosate-resistant S. halepense. Copyright © 2011 Society of Chemical Industry

Item Type: Article
Uncontrolled Keywords: glyphosate leaf uptake; glyphosate translocation; perennial Johnsongrass weed; non-target-site resistance mechanism
Author Affiliation: Universidad de Buenos Aires, Buenos Aires, Argentina, Monsanto Argentina, Buenos Aires, Argentina,Instituto Nacional de Tecnología Agropecuaria (INTA Castelar), Hurlingham, Argentina, Universidad de Buenos Aires, Buenos Aires, Argentina, Institute of Agriculture, University of Western Australia
Subjects: Plant Production
Plant Protection
Divisions: Sorghum
Depositing User: Mr. SanatKumar Behera
Date Deposited: 04 Sep 2012 13:40
Last Modified: 04 Sep 2012 13:42
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