Effect of a novel mutation in a D9-stearoyl-ACP-desaturase on soybean seed oil composition

Ruddle II, P. and Whetten, R. and Cardinal, A. and et al, . (2012) Effect of a novel mutation in a D9-stearoyl-ACP-desaturase on soybean seed oil composition. TAG Theoretical and Applied Genetics. pp. 1-9.

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Soybean [Glycine max (L.) Merr.] oil typically contains 2–4 % stearic acid. Seed oil with 20 % stearic acid would be useful for solid fat applications, both for its cooking properties and health benefits. Breeding lines with high stearic acid have been developed, but many suffer from agronomic problems. This study identifies a new source of high stearic acid, determines its relationship with another high stearic locus and presents molecular markers for it is use in breeding. TCJWB03-806-7-19, a ‘Holladay’ mutant with high stearic acid, was crossed to two FAM94-41-derived lines that contained a point mutation in a seed-specific isoform of a Δ9-stearoyl-acyl carrier protein-desaturase (SACPD-C). Fatty acid analysis was performed over two growing seasons with F 2-derived lines and transgressive segregation for stearic acid content was observed. Sequencing of SACPD isoforms in TCJWB03-806-7-19 revealed the deletion of an ‘A’ nucleotide in exon 3 of SACPD-B, which results in a protein whose final 28 amino acids are predicted to differ from Williams 82 SACPD-B. Sorting intolerant from tolerant (SIFT) analysis revealed that this frameshift mutation may affect SACPD-B protein function. Allele-specific genotyping for the SACPD-C point mutation and SACPD-B nucleotide deletion was performed in both populations. Additive effects and R 2 for stearic acid were +3.3 and 0.55 for SACPD-C and +1.9 and 0.19 for SACPD-B. Average stearic acid in lines homozygous for both mutations was 14.6 %. This SACPD-B mutation represents a novel high stearic allele.

Item Type: Article
Additional Information: This research was supported in part by the United Soybean Board and the North Carolina Soybean Producers Association.
Author Affiliation: Department of Crop Science, North Carolina State University, Box 7620, Raleigh, NC 27695, USA
Subjects: Crop Improvement
Divisions: Soyabean
Depositing User: Mr Siva Shankar
Date Deposited: 12 Sep 2012 05:00
Last Modified: 12 Sep 2012 05:02
Official URL: http://dx.doi.org/10.1007/s00122-012-1977-5
URI: http://eprints.icrisat.ac.in/id/eprint/7838

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