A single medium for the isolation of acetylene-reducing (dinitrogen-fixing) bacteria from soils

Rennie, R.J. (1981) A single medium for the isolation of acetylene-reducing (dinitrogen-fixing) bacteria from soils. Canadian Journal of Microbiology, 27 (1). pp. 8-14.

[img] PDF - Published Version
Restricted to ICRISAT researchers only

Request a copy


A single medium containing standard basal salts and three common carbon sources (sucrose, mannitol and sodium lactate) is proposed to replace nitrogen-free media in common use for isolating dinitrogen-fixing bacteria. Eight commonly isolated genera of dinitrogen-fixing bacteria exhibited growth on this combined carbon medium that equalled or bettered growth on other carbon-containing media. Combined carbon medium also yielded the highest counts of putative dinitrogen-fixing bacteria from three southern Alberta soils. In a survey of the bacteria isolated aerobically from the Burdett soil on combined carbon agar 75% of the isolates exhibited acetylene reduction. These bacteria were identified as Azospirillum spp., Bacillus polymyxa, B. macerans, Klebsiella pneumoniae, Erwinia herbicola and Enterobacter cloacae. The inclusion of yeast extract in combined carbon medium is considered essential to supply organic growth factors and may supply starter nitrogen which promotes growth without inhibiting acetylene reduction

Item Type: Article
Author Affiliation: Research Station, Agriculture Canada, Lethbridge, Alta., Canada T1J 4B1
Subjects: Soil Science and Microbiology
Divisions: General
Depositing User: Mr B Krishnamurthy
Date Deposited: 01 Oct 2012 06:52
Last Modified: 01 Oct 2012 06:52
Official URL: http://dx.doi.org/10.1139/m81-002
URI: http://eprints.icrisat.ac.in/id/eprint/8143

Actions (login required)

View Item View Item