High Frequency Somatic Embryogenesis in Cotton

Aydin, Y. and Ipekci, Z. and Talas-Oğraş, T. and et al, . (2012) High Frequency Somatic Embryogenesis in Cotton. Biologia Plantarum , 48 (4). pp. 491-495.

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A highly reproducible system for efficient somatic embryogenesis was developed to regenerate plantlets from cotton (Gossypium hirsutum L.) cultivars (Nazilli M-503 and Nazilli 143). Shoot apices, hypocotyls and nodes of 10-d-old seedlings were used as explants. High frequency (100 %) embryogenic calli was initiated from all tested explants on Murashige and Skoog (1962) (MS) media supplemented with 1 g dm–3 polyvinylpyrrolidone (PVP), 1 mg dm–3 6-benzylaminopurine (BAP), 0.5 mg dm–3 kinetin for Nazilli M-503 and 1 g dm–3 PVP, 2 mg dm–3 BAP, 0.5 mg dm–3 kinetin for Nazilli-143. Globular stage somatic embryos were produced 4 months after transfer to hormone-free MS medium supplemented with 1 g dm–3 PVP. Subsequent subculture of globular embryos every 3 weeks on hormone-free MS medium led to the development of torpedo and cotyledonary stage embryos with the frequency of 75 and 83.2 % from hypocotyl explants of Nazilli M-503 and Nazilli-143, respectively. Afterwards, mature somatic embryos were isolated and cultured on hormone-free MS medium for germination and development into plantlets. The highest germination frequency (42.9 %) for Nazilli M-503 somatic embryos were obtained on hormone-free MS medium after 5 months with hypocotyl explants, whereas germination frequencies of Nazilli-143 embryos from hypocotyl, node and apex explants varied between 22 – 30 %.

Item Type: Article
Uncontrolled Keywords: Gossypium hirsutum L. - indirect somatic embryogenesis - hypocotyls - cotyledonary node - apex Fulltext Preview
Author Affiliation: Research Institute for Genetic Engineering and Biotechnology, TUBITAK, Marmara University,Institute for Genetic Engineering and Biotechnology,Istanbul University
Subjects: Soil Science and Microbiology
Plant Physiology and Biochemistry > Biochemistry
Depositing User: Mr. SanatKumar Behera
Date Deposited: 04 Oct 2012 15:10
Last Modified: 04 Oct 2012 15:10
Official URL: http://dx.doi.org/10.1023/B:BIOP.0000047142.07987....
URI: http://eprints.icrisat.ac.in/id/eprint/8256

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