Effect of Growth Regulators on Peanut “Florman Inta” Regeneration

Faustinelli, P.C. and Racca, R.W. and Collino, D.J. and et al, . (2009) Effect of Growth Regulators on Peanut “Florman Inta” Regeneration. Peanut Science, 36 (2). pp. 138-143.

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Tissue culture is a necessary tool in the genetic modification of peanut for the improvement of its agronomic and nutritional attributes. Since genotype can affect tissue culture responses, the main objective of this research was to determine the optimum concentration of auxins and cytokinins in the basal media needed for organogenesis from Arachis hypogaea L. cv. Florman INTA. The first two leaves (2–5 mm in length) were dissected from aseptically germinated seeds and cultivated on Murashige and Skoog (MS) medium supplemented with 16 combinations of α-naphthaleneacetic acid (NAA) (0.01 and 1 mg/l) and benzyladenine (BAP) or kinetin (KIN) (1 to 10 mg/l) during the initiation stage. Bud regeneration occurred in all growth regulator combinations, but the maximum number of buds per explant (1.2) was regenerated at 1 mg/l NAA with 3 mg/l BAP. Development of buds into shoots was readily achieved by transferring regenerated buds onto fresh medium containing 0.01 mg/l NAA (without BAP). Roots were induced to grow when shoots were transferred to medium containing 3 mg/l of NAA. The vigorous root system allowed for a high survival rate of the plantlets after transplanting. The overall efficiency of the system was 15%. Plants transplanted into soil were completely normal and capable of producing seeds

Item Type: Article
Uncontrolled Keywords: Arachis hypogaea, in vitro culture, regeneration, organogenesis
Author Affiliation: Agric. Engineer (M.Sc.) Faculty of Agricultural Sciences, Catholic University of Córdoba, Córdoba, Argentina
Subjects: Plant Production
Crop Improvement
Divisions: Groundnut
Depositing User: Ms K Syamalamba
Date Deposited: 05 Oct 2012 04:55
Last Modified: 05 Oct 2012 04:55
Official URL: http://dx.doi.org/10.3146/PS07-012.1
URI: http://eprints.icrisat.ac.in/id/eprint/8263

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