Further studies on plantlet production from cultured tissues of Sorghum bicolor

Dunstan, D.I. and Short, K.C. and Dhaliwal, H. and Thomas, E. (1979) Further studies on plantlet production from cultured tissues of Sorghum bicolor. Protoplasma, 101 (4). pp. 355-361.

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Cultures capable of continuous plantlet production were established from excised immature embryos of sorghum and the course of development of the plantlets was followed by light and scanning electron microscopy. Such analyses revealed that there were 2 distinct methods of plantlet production. Shoot primordia and embryo-like structures arose de novo from cells of the scutellum. However, when these cultures were transferred to fresh medium a further proliferation of shoot buds occurred through the formation of axillary shoot primoridia. The cultures were therefore more comparable to shoot cultures than to callus cultures. Over 300 plantlets produced in vitro were transferred to potting compost and grown to maturity. Most plants flowered and set seed; 15 plants were sterile but were of normal chromosome number (2n = 20), and it is presumed that the sterility was due to segregation of restorer genes in the immature embryos used to initiate some of the cultures

Item Type: Article
Author Affiliation: Department of Biology, Division of Plant Science, North East London Polytechnic, London E15 4L2, UK
Subjects: Crop Improvement > Genetics/Genomics
Crop Improvement > Plant Breeding
Divisions: Sorghum
Depositing User: Ms K Syamalamba
Date Deposited: 05 Oct 2012 10:41
Last Modified: 05 Oct 2012 10:41
Official URL: http://dx.doi.org/10.1007/BF01276974
URI: http://eprints.icrisat.ac.in/id/eprint/8289

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