Change in membrane fatty acid compositions and cold-induced responses in chickpea

Shahandashti, S.S.K. and Amiri, R.M. and Zeinali, H. and Ramezanpour, S.S. (2012) Change in membrane fatty acid compositions and cold-induced responses in chickpea. Molecular Biology Reports. pp. 1-11.

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Plant cells often increase cold tolerance by reprogramming their genes expression which results in adjusted metabolic alternations, a process enhanced under cold acclimation (CA) phase. In present study, we assessed the changes of membrane fatty acid compositions and defense machine (like antioxidative enzymes) along with damage indexes like electrolyte leakage index (ELI) and malondialdehyde (MDA) during CA, cold stress (CS) and recovery (R) phases in chickpea (Cicer arietinum L.). Results showed an increase in unsaturated fatty acids ratio compare to saturated ones which is a sign of cold tolerance especially after CA phase. Antioxidant enzymes had an important role during CA and R phases while CS affected their activity which can be a sign for associating other metabolites or enzymes activities to create cold tolerance in plants. To investigation of enzymes assay under experimental treatments, the expression pattern of some enzymes including superoxide dismutase (sod), catalase (cat) and lipoxygenase (lox) was studied using quantitative real time PCR. LOX activity has shown a bilateral behavior: a positive relation with membrane damage index in CA and an interesting link with double bond index (DBI) in CS indicating probably its role in secondary metabolites like jasmonic acid signaling pathway. It was suggested that increased DBI and low LOX activity under CS could be a reason for plant cold tolerance.

Item Type: Article
Uncontrolled Keywords: Chickpea – Cold acclimation – Cold responses – Fatty acids – Gene expression
Author Affiliation: University College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran
Subjects: Crop Improvement
Plant Physiology and Biochemistry > Biochemistry
Divisions: Chickpea
Depositing User: Mr. SanatKumar Behera
Date Deposited: 22 Oct 2012 07:36
Last Modified: 22 Oct 2012 07:36
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