Isolation and characterization of lipopeptide antibiotics produced by Bacillus subtilis

Chen, H. and Wang, L. and Su, C.X. and et al, . (2008) Isolation and characterization of lipopeptide antibiotics produced by Bacillus subtilis. Letters in Applied Microbiology, 47 (3). pp. 180-186.

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Aims:  Antibiotics from Bacillus subtilis JA show strong pathogen inhibition ability, which has potential market application; yet, the composition of these antibiotics has not been elucidated. The aim of this paper is to isolate and identify these antibiotics. Methods and Results:  The antagonistic activity of JA was tested in vitro; it exhibited strong inhibition against some important phytopathogens and postharvest pathogens. Crude antibiotic production was extracted with methanol from the precipitate by adding 6 mol l−1 HCl to the bacillus-free culture broth. The crude extract was run on Diamonsil C18 column (5 μm, 250 × 4·6 mm) in HPLC system to separate the antibiotics. Major antibiotics were classified into three lipopeptide families according to electrospray ionization–mass spectrometry analysis. Subsequently, the classification of antibiotics was confirmed with typical collision-induced dissociation fragments. Conclusions:  Three kinds of antibiotics were isolated from B. subtilis JA and were identified to the lipopeptide families, surfactin, iturin and fengycin. These compounds could function as biocontrol agents against a large spectrum of pathogens. Significance and Impact of the Study:  This study provided a reliable and rapid method for isolation and structural characterization of lipopeptide antibiotics from B. subtilis

Item Type: Article
Uncontrolled Keywords: Antibiotic; Bacillus subtilis; characterization; electrospray ionization mass spectrometry; lipopeptide
Author Affiliation: Key Laboratory of Ion Beam Bioengineering, Chinese Academy of Sciences, Hefei, Anhui, China
Subjects: Plant Protection
Plant Physiology and Biochemistry > Plant Physiology
Plant Physiology and Biochemistry > Biochemistry
Divisions: Other Crops
Depositing User: Ms K Syamalamba
Date Deposited: 29 Oct 2012 05:15
Last Modified: 29 Oct 2012 05:15
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