Structural studies on the water-soluble gums from the endosperm of sorghum grain

Woolard, G.R. and Rathbone, E.B. (1977) Structural studies on the water-soluble gums from the endosperm of sorghum grain. Carbohydrate Research, 53 (1). pp. 95-100.

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Purification by Bio-Gel P-100 chromatography of the cold-water-soluble gum from the endosperm of sorghum grain gave an electrophoretically homogeneous d-glucan. This polysaccharide (glucan A) had [α]d20 + 88° and 23. Methylation analysis and periodate-oxidation studies indicated that glucan A has both (1→4)- and (1→6)-linked d-glucopyranose residues, in the ratio of 2:1. On average, three d-glucose residues per molecule of glucan A are branched. Chromium trioxide oxidation analysis of glucan A indicated a preponderance of α-d-glucosidic linkages. P.m.r. spectroscopy of permethylated glucan A showed the presence of α- and β-linkages in the ratio of 4:1. Purification of the hot-water-soluble gum by Bio-Gel P-100 chromatography gave glucan B, having [α]d20 + 134° and 20. Structural studies showed glucan B to be similar to glucan A, differing in the ratio of (1→4)- to (1→6)-linkages; glucan B has these d-glucosidic linkages in the ratio of 6:1

Item Type: Article
Author Affiliation: National Chemical Research Laboratory, Council for Scientific and Industrial Research, Pretoria 0001 South Africa
Subjects: Crop Improvement > Seed Technology
Divisions: Sorghum
Depositing User: Ms K Syamalamba
Date Deposited: 11 Dec 2012 05:18
Last Modified: 11 Dec 2012 05:18
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