Simple Sequence Repeat Markers Developed from Maize Sequences Found in the GENBANK Database: Map Construction

Senior, M.L. and Chin, E.C.L. and Lee, M. and et al, . (1996) Simple Sequence Repeat Markers Developed from Maize Sequences Found in the GENBANK Database: Map Construction. Crop Science, 36 (6). pp. 1676-1683.

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Simple sequence repeats (SSRs) are rapidly becoming an important class of DNA markers that are being widely used to map both plant and animal genomes. SSRs have the advantage of providing a codominant marker system based on polymerase chain reaction (PCR) methodology. Although the presence of SSRs is now well documented in the plant kingdom, a mapped set of primer sequences in maize (Zea mays L.) is not available. Polymorphic primer pairs developed from maize sequences in GENBANK were mapped to 42 loci in maize by means of either a B73 × Mo17, Mo17 × H99, or B73 × G35 recombinant inbred population. All SSR loci were found to be linked to one or more adjacent restriction fragment length polymorphism (RFLP) and/or isozyme loci. Segregation followed a pattern of Mendelian inheritance with one SSR locus deviating from expected ratios at a 1% level of significance. The SSRs were distributed throughout the maize genome with no evidence of clustering. Each SSR marker detected a single locus.

Item Type: Article
Additional Information: This work is a collaborative effort between the USDA-ARS, Raleigh, NC, and Pioneer Hi-Bred International, Johnston, IA, and was in part funded by the NC Biotechnology Center through Institutional Development Grant No. 9110-IDG-1022 and the United States Department of Agriculture through NRICGP Grant No. 94-37300-0296 to M.L. Senior and also by IAEA contract no. 8494/RO and the Iowa Agricultural and Home Economics Experiment Station Project 3134 to M. Lee.
Author Affiliation: DA-ARS, Molecular Marker Facility, Box 7620, North Carolina State Univ., Raleigh NC 27695
Subjects: Crop Improvement
Divisions: Maize
Depositing User: Mr Siva Shankar
Date Deposited: 12 Dec 2012 05:15
Last Modified: 12 Dec 2012 05:15
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